Library
Contact UsCellCiphr® Profiles
CellCiphr® Profiles are derived by evaluating the systemic response of a Cellular Model of Disease to a systemic modulator such as a small molecule, peptide or environmental toxin. These Cellular Models of Disease are engineered to "measure and manipulate" the cellular system to better reflect disease (dys)function. Multiple features of the cellular systemic response (6 or more) are captured simultaneously. These measurements are compiled to create a CellCiphr® Profile. CellCiphr® Profiles can be constructed to facilitate rapid-response pattern identification (Heat Maps), subtle dose-dependent effects (Distribution Maps) and connections between cellular processes (Cell Maps). CellCiphr® Profiles also form the foundation of higher-order analyses to classify response patterns by chemotype or common mechanism of action.
- CSB™ Assays
- drugs
- cellular targets
- cell types
- Heat Maps for Fast Identification of Patterns
- Distribution Maps for Population Average in Large Data Sets
- Cell Maps Decipher Connections between Cellular Processes
Cellumen has created a profile of gene switched hdm2 expression in A549 cells. Up-regulation of cellular hdm2 expression impacts many cellular processes, with variable efficacy. Cellumen used fluorescence-based reagents to profile more than 10 cellular parameters.
CellCiphr® profile of gene switched hdm2 expression in A549 cells
Cellumen has used the CellCiphr® Gene Switched technology to manipulate the expression of mutant huntingtin protein, which forms aggregates that may be causative in the progression of Huntington's Disease. Cellumen scientists monitored aggregate formation in a "switched" PC12 line over several days and developed methods to quantify the number, distribution, and morphology of the aggregates. Using CellCiphr® Profiles, multiplexed assay data of the aggregation process revealed several cellular systemic responses that are specific to aggregate-containing cells. The curves below indicate the average response of a population of cells stimulated to express mHtt: as aggregate number increases (black line), cells die (purple line). A Cell Map analysis 3 and 48 hours after induction of mHtt expression reveals that stress responses (c-Jun activation, Y axis of inserted Cell Map) are apparent only in the cells containing aggregates (X axis of Cell Map). The mHtt stress response profile is expressed in the Cell Map by the appearance of a new population of cells shifted to the right (containing aggregates) and above (c-Jun activation) of the untreated population.
