Library
Contact UsCellCiphr® Gene Switched Huntingtin Protein
Cellumen uses a tight gene switch to create reagents that tightly regulate the expression of either proteins or siRNAs. Multiple gene switches in the same cells permit more sophisticated manipulations of cellular processes. Expression of proteins that may be cytotoxic or cytostatic can be manipulated to enable Cellular System Biology profiling in a controlled manner.
Example: CellCiphr® Gene Switched Huntingtin Protein
CellCiphr® Gene Switched Protein technology enabled Cellumen to manipulate the expression of mutant huntingtin protein, which forms aggregates that may be causative in the progression of Huntington’s Disease. Aggregate formation was monitored in a "switched" PC12 cell line over several days. CellCiphr® Profiles and CSB™-based assay data of the aggregation process revealed several cellular systemic responses that are specific to aggregate-containing cells.
This image set represents a stable PC12 cell line expressing full-length Huntingtin with a 73 glutamine repeat fused to red fluorescent protein under the control of the ecdysone-inducible promoter. There is a very low level of Htt (Huntingtin) expression in the un-induced cells (Control). After 48 hours of exposure to the ecdysone receptor ligand, Htt is expressed at high levels (Induced).
Htt-expressing cell line was the generous gift of L. Thompson and CHDI
CellCiphr® Gene Switched Protein technology enabled Cellumen to manipulate the expression of mutant huntingtin protein, which forms aggregates that may be causative in the progression of Huntington’s Disease. Aggregate formation was monitored in a "switched" PC12 cell line over several days. CellCiphr® Profiles and CSB™-based assay data of the aggregation process revealed several cellular systemic responses that are specific to aggregate-containing cells.
Htt-expressing cell line was the generous gift of L. Thompson and CHDI