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Contact UsCellular Systems Biology – CellCiphr® Toxicity Panel 1 and Panel 2
CellCiphr® Toxicity Profiling uses relevant tissue cell types, a High Content reader, and panels of fluorescent reagents to illuminate cellular responses to toxic challenges in a specially designed set of fixed end-point assays. CellCiphr® Panel 1, a two-plate assay conducted in HepG2 cells, captures 10-point dose-response data from 10 cellular parameters, each performed at 1, 24 and 72 hours, to create a 30-cell-feature, time- and concentration-dependent profile. CellCiphr® Panel 2, a two-plate assay using primary rat hepatocytes, captures 10 point dose-response data from 8 cellular features, each performed at 1, 24 and 48 hours.
| CellCiphr® Toxicity Panel 1 HepG2 |
CellCiphr® Toxicity Panel 2 Rat Hepatocytes |
|
Cell Loss Nuclear Size Cytoskeletal Disruption DNA Damage Response Oxidative Stress Mitosis Marker Stress Kinase Activation Mitochondria Function I Mitochondria Function II Cell Cycle Arrest |
Cell Loss Nuclear Size DNA Damage DNA Fragmentation Apoptosis Phospholipidosis Steatosis Mitochondria Function |
CellCiphr® Toxicity Panel 1 and CellCiphr® Toxicity Panel 2 assays evaluate compound effects over a broad-range of indicators.
Using CellCiphr® Toxicity Panels
CellCiphr® Toxicity Panels 1 and 2 are designed to be used as stand-alone
assays or in combination to provide a more detailed profile. The panels are performed
in 384 well microtiter-plates using a broad concentration range and 3 time points with
extensive intra- and inter-plate quality control, to give researchers:
- Measures of potency in critical toxicity endpoints
- Detailed profiles of multiple toxicity mechanisms
- Identification of sub-lethal effects on cell health and function
- Cause and effect through dose and time dependent measures on critical cell functions
Example of images collected from the two plate CellCiphr® Toxicity Panel 1 HepG2 assays. False colors are added to illustrate sub-cellular compartments or effects. Plate 1 - nucleus (a), oxidative stress (b), stress kinase (c), DNA damage (d); and, Plate 2 – nucleus (e), mitosis marker (f), mitochondria (g), microtubules (h).
The data collected by HCS readers is used to fit a dose-response curve for the cell feature and an AC50 value is calculated. The collection of AC50 values from the 30 cell features in Panel 1, or 24 cell features in Panel 2 are used to generate the compound profile using CellCiphr Profiles.
Screen compounds for a broad range of potentially toxic effects early in the drug discovery process with CellCiphr® Toxicity Panels. Contact us to learn more.