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Contact UsCellular Systems Biology – CellCiphr® Panel 1 and Panel 2
CellCiphr® Cytotoxicity Profiling uses relevant tissue cell types, a high content reader and panels of fluorescent reagents to illuminate cellular responses to toxic challenges in a specially designed set of fixed end point assays. CellCiphr® Panel 1, a two-plate assay conducted in HepG2 cells, captures 10-point dose-response data from 11 cellular parameters, each performed at 1 hr, 1 and 3 days, to create a 33-cell-feature, time- and concentration-dependent profile. CellCiphr® Panel 2, a two-plate assay using primary rat hepatocytes, captures 10 point dose-response data from 10 cellular features, each performed at 1 hr, 1 and 2 days.
| CellCiphr® Panel 1 HepG2 |
CellCiphr® Panel 2 Rat Hepatocytes |
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Cell Loss DNA Degradation Nuclear Size Cytoskeletal Disruption DNA Damage Response Oxidative Stress Mitosis Marker Stress Kinase Activation Mitochondria Function I Mitochondria Function II Cell Cycle Arrest |
Cell Loss DNA Degradation Nuclear Size Cytoskeletal Disruption Peroxisomal Proliferation Phospholipidosis Stress Kinase Activation Apoptosis Mitochondria Function I Mitochondria Function II |
CellCiphr® Panel 1 and CellCiphr® Panel 2 assays evaluate compound effects over a broad-range of indicators.
Using CellCiphr® Cytotoxicity Panels
CellCiphr® Cytotoxicity Panels 1 and 2 are designed to be used as stand-alone assays or in combination to provide a more detailed profile. The panels are performed in 384 well microtiter-plates using a broad concentration range and 3 time points with extensive intra- and inter-plate quality control, to give researchers:
- Measures of potency in critical cytotoxicity endpoints
- Detailed profiles of multiple cytotoxicity mechanisms
- Identification of sub-lethal effects on cell health and function
- Cause and effect through dose and time dependent measures on critical cell functions
Example of images collected from the two plate CellCiphr® Panel 1 HepG2 assays. False colors are added to illustrate sub-cellular compartments or effects. Plate 1 - nucleus (a), oxidative stress (b), stress kinase (c), DNA damage (d); and, Plate 2 – nucleus (e), mitosis marker (f), mitochondria (g), microtubules (h).
The data collected by HCS readers is used to fit a dose-response curve for the cell feature and an AC50 (Activity Concentration at which ½ the cells respond) value is calculated. The collection of AC50 values from 33-cell features are used to generate the compound profile using CellCiphr Profiles.
Screen compounds for a broad range of potentially toxic effects early in the drug discovery process with CellCiphr® Cytotoxicity Panels.